384-Lane Parallel Microfluidic Cytometer

Under NIH funding we are now developing a novel 384-channel parallel microfluidic cytometer (PMC) that is capable of high-content (image-based) and rare-cell assays. This system will have more than ten-times the sample throughput of microscope-based imaging systems and may help to move cell-based assays to new applications in drug discovery, genomics, and cancer research.

 

 

 

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Detail of the confocal scanner used as a detector on the PMC.

 

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PMC – A 384-channel microfluidic plate for parallel microfluidic cytometery

 

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Results for a single run of a 384-lane PMC. Dilution curve study

 

 

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Plan view of a 384-channel microfluidic cytometer plate and spinning confocal detector

 

Mckenna BK, Salim H, Bringhurst FR and Ehrlich DJ; 384-Channel Parallel Microfluidic
Cytometer for Rare-Cell Screening, Lab on a Chip 9, 305-310, 2009.

Ehrlich, DJ, McKenna, BK, Evans, JG, Belkina, AC, Denis, GV, Sherr, DH and Cheung MC;
Parallel Imaging Microfluidic Cytometer, Methods in Cell Biology 102, 49-75, 2011

Bringhurst FR and Ehrlich DJ; 384-Channel Parallel Microfluidic Cytometer
for Rare-Cell Screening, Lab on a Chip 9, 305-310, 2009.