Nucleic acid and protein mass mapping by live-cell deep-UV microscopy

Boston University Cell Photometrics Laboratory













Quantification of nucleic acid and protein content in fixed and live cells


Background subtracted, raw optical density images

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Nucleic acid and protein mass maps compared to nucleic acid stains

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Distribution of protein and nucleic acid mass compared to nucleic acid dyes

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Mass maps can reveal details, comparable to staining, at 100x magnification

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Cheung, MC, Evans,JG, McKenna, BK, and Ehrlich, DJ, Deep Ultraviolet Mapping of Intracellular Protein and Nucleic Acid in Femtograms per Pixel, Cytometry A, 79A, 920-932, 2011.

Zeskind BJ, Jordan CD, Timp W., Trapani L, Waller G, Horodincu V, Ehrlich DJ, and Matusudaira P: Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy. Nature Methods 4(7); 567-569, 2007.